This is a full guide to the NG 100% run, which includes proposed rules, a list of all required items, and a route including maps showing locations of all the required items. Splits to go along with this guide are available in the Discord.

https://docs.google.com/document/d/1THtCUGD-9978SoZCms3zvYQJyJtdXFdFczUIacpPl4A/edit?usp=sharing

Updated 5/30/21: changed some routing order. New route has you get broken slab after Beneviento, then proceed to Moreau. After you acquire Grenade Launcher and Wolfsbane, do East Old Town treasures, Lone Road Boat Rides, get the speed boost from Duke, THEN return the slab to Beneviento grave so that you have Wolfsbane to use on Axe guy. Also changed route slightly after getting ball and key mold to be more optimized use of time.