If you haven't already, take a look at Znernicus's tutorial series on youtube. It goes through routes chapter by chapter. There are a few chambers that have new routes, but for just starting out it's super helpful.
Also, not sure how much you've gotten into speedrunning Portal 2 yet, but bunnyhopping is pretty much necessary for movement. The best way to get better at it is to just bite the bullet and practice. Invictus has a good tutorial on how it works.
Those are the most important things I can think of right now.
Very informative Znern. I know you always endorsed the Live Timer but didn't realize the inaccuracies were this big. I think the 30 seconds penalty is extremely fair. Currently, I'm dying enough during single segments that P2LT would be a huge pain, so I'll just eat the 30 seconds.
Thanks for the information, though.