WALLJUMPING IS NOW PATCHED, SO THIS STRATEGY WILL NOT WORK ANYMORE!
This is the fastest known strategy for a Pods speedrun. If you're lucky, and everything goes as planned, you might even be able to do a 36-second run.
Easiest to do with an Assault with at least 100% movement, 25 points in Adrenaline and a really good gun, like the Shockfield.
You can't always get the easiest possible boss, so you need a bit of luck, if you want to save a few seconds.
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Buy the "Killing Machine" powerup for some extra damage.
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Once the game starts, activate your Adrenaline and immediately run to the wall, that should break itself in about 20 seconds. This can be avoided by using a glitch called "walljump", which basically allows you to run through certain walls and obstacles. You need to be really fast to do it.
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Once you've jumped through the wall, run to the down-right corner of the room. Don't care about the purge nests, leave them alone. You should be able to run through the down-right part of the wall.
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After that, run into the middle of the boss room. That's the place where the boss egg should spawn. Zombie eggs only spawn when you come near the boss room gate, so there are no eggs yet. Place down 2 or 3 flame turrets depending on your mastery level. The turrets should be right where the boss egg spawns.
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Run to the right, where the boss room gate is. That should make all the eggs spawn. Don't care about other eggs, run straight to the boss egg
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The turrets will automatically start shooting the boss egg, even though it isn't a zombie. When turrets are inside of the boss egg, the game thinks that the egg is a zombie, so they start shooting, breaking the egg really fast.
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Once the boss egg breaks, start shooting the boss(es) to kill them as fast as possible. Once the boss(es) is/are killed, the countdown will start. Wait for the countdown to end, the game will end after that, and you'll see your time.