Start the run once selecting "normal" mode.

Escape: Is split once exiting the early game dark hallway.

Black Latex Hall 1: Is split once exiting the first Black Latex puzzle.

Black Latex Hall 2: Is split once entering Dragon room.

Dragon: Is split once exiting Dragon room.

Meet Puro: Is split once entering his room after evading Tail Monster.

Sit with Puro: Is split once selecting to sit with Puro.

Dark Maze & Workshop: Is split once entering Workshop.

Gas Room: Is split once entering the room where Puro helps you avoid Pup enemy.

Generator 1 & 2: Is split once activating the second generator in Shark Boy room.

Shark Boy: Is split once exiting Shark Boy room.

Squid Dog: Is split once getting the last hit on Squid Dog.

White Latex Room 1 & 2: Is split once leaving the second White Latex room.

Lion Chase: Is split once leaving second White Latex room.(Or once defeating Lion.)

Lab Escape: Is split once escaping the lab and entering the balcony room again.

White & Black Latex Hall: Is split once entering the room before the End room.

End: Is split once you see Puro's face appear on the True Good ending screen.