I want to do 100-110 run, but that takes a lot of hours and i can't do that in a single segment. It may take me ~9 hours and i have things to do IRL

Can my run be segmented?

Edit: Also i use Zygor guide addon, there is no problem with that?

Usually I would like full runs, but if you can include the load in screen and /played when you start a new segment and when you end a segment, and stitch all the videos together, that would probably work. And yes, zygor is fine as long as it does not perform any actions for you such as turning in a quest or auto picking a flight point.