Easiest way to do this run is on a DS that doesnt have a save file. Timing starts when you press any button at the start screen Listed below (as the route) are the characters you unlock and how to unlock them, you are not allowed to user versus mode to get characters (example: in order to get Ness you cant do 10 matches you have to Clear Classic Mode on any difficulty)

Bowser Jr. - As Bowser, clear Classic Mode on intensity 6.0 or higher Ganondorf - As Zelda or Link, clear Classic Mode on intensity 5.0 or higher Dr. Mario - As Mario, clear Classic Mode on intensity 4.0 or higher (Use Ganondorf) Dark Pit - Clear Classic Mode with three or more characters Lucina - As Marth, clear Classic Mode without any continues (Use Captain Falcon) Falco - Clear Classic Mode without any continues (Use Rosalina) Ness - Clear Classic Mode on any difficulty (Use Ness) Duck Hunt - Clear Classic Mode with 8 or more characters (Use Dedede & Ike) Mr. Game & Watch - Clear Classic Mode with 10 or more characters Wario - Clear 100 Man Smash Jigglypuff - Collect at least 30 different equipment items R.O.B. - Collect at least 200 different trophies