Advancing frame by frame with YouTube's keyboard shortcut you can see first Princess' frame at 25m 11s 170ms.

I use VisualBoyAdvance 1.7.2 with "Super Mario World Super Mario Advance 2 [J]" ROM.

I time my runs manually with LiveSplit and I use a generic USB controller (SNES shaped) all buttons are mapped as they should while X and Y, are used to control LiveSplit as follows: X - Starts/Splits. Y - Resets timer.

I use NintendoSpy as Input Display with a custom skin. The XML archive doesn't recognize my D-pad as a D-pad but as a Stick instead and that's why when I'm not pressing any button the red circle lights in the center as if it were a stick.