To begin with this is a Guide for the Category "Beat the Game (Co-Op/Solo)"
The optimal RNG Boss Order should look like this :
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Eternal Star (The Skytemple Stage can Kill your run Really Really Quick, thanks to the environmental Hazards)
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Kur (Same reason as Enternal Star)
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The Perforator (Eventough you have already Killed Kur take his Weapon because it doesn't trigger i-frames, will be useful later on)
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Twin Astrals (if get them on higher levels you will have to play an Anoying "Dodge the Bullets game")
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Rollster (Astrals weapon is super Powerful angainst him)
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Death_Lotus (Use the "Gattlin gun" and D-Boost if goes in to his "Turtle Stance" since he does not Spawn any Plant minions anymore)
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Vile Visage (Always try to Hit his Weakspot)
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Shatterbreak (no threat at all xD)