I see a lot of runs with the disc versions, I have it on PSTV (VitaTV) off of PSN, and it likely runs faster because of the flash memory. Is the disc version required to run?

This is a mini-guide for NG+. Treat this like a work in progress and feel free to add input!

The route is almost exactly the same except you should not pick up anything besides essential items (keys, fuses, etc)

You want to take as few items from fights as possible in order to avoid item dumps. Train yourself to not take any items – the button pattern is different for level ups so I will be noting all level ups in the run that are standard. Before day 3, all level ups in the run are predictable. Day 3 starts the potential re-fights, so you need to watch your own EXP to make sure you are dumping items predictably when you level up.

Day1

As soon as you have control of Aya, equip the Gun and Vest from your EX save file. Put all the bonus points into BP and Inventory space.

(this can be done without putting BP into inventory, but it will be more difficult.)

The fights you have to do in Day1 typically are only ammo, but rarely other items appear. I recommend just spamming X and taking everything from fights in day 1 except for the vest from gator.

LevelUP! - first sewer fight

LevelUP! - gator fight

Day 2

NYPD→Museum hit RIGHT ONCE Museum → NYPD hit LEFT ONCE

Central Park (CP)

LevelUP! - 1st CP fight w/snakes

LevelUP! - 3rd CP fight with birds and monkey thing

LevelUP! - 4th CP fight before gate

LevelUP! - 6th CP fight with bear

LevelUP! - 8th CP fight right before worms

LevelUP! - worms. The best way to do the worms fight is to start firing the SECOND the third worm pops. once they are dead, immediately kill the 4th worm.

Day 3

SOHO → Museum hit LEFT TWICE Museum → NYPD hit LEFT TWICE

Day 4

NYPD → Hospital hit LEFT TWICE

Day 5

NYPD → Chinatown hit LEFT THREE TIMES

Something to note here, if you haven't filled inventory from this point, the best strat is to just take all items, and discard just one after the centipede fight. The gate key is the last required item that will not let you take it unless you have a spot free.

Subway → Museum hit LEFT OR RIGHT FIVE TIMES

Once you get the Klamp Key you can smash X through all fights, as you no longer need any essential items. If your items are full coming into the lab, the game will force you to take the gun and the key. This is faster than doing an inventory dump beforehand.

Good Luck! Please comment below if you have any ideas or tips to add to this guide.