Hey there, I know I'm still new, but I've been running the game a lot the last few days and watching various Pre-1.3.2 runs I am noticing some changes that are forcing the runs to be longer. I've done my best to time them out and present them all here.

Level ending directly from the boss room as opposed to having to run to another room and take a teleporter back

Amsusias Run: Forced Interaction with Leshy that takes roughly 13 second, Interaction with Leshy and Amdusias' Transformation adding 12 to 15 seconds

Valefar Run:Forced 8 second Leshy interaction WITH an additional multi wave battle that wasn't required before. An additional interaction and teleport out that adds another 15 seconds.

Barbatos Run: Another unskippable interaction with the Three Bishops that adds 15 seconds of unmovable time AND another additional multi wave battle.

Leshy Run: Another 12 second unskippable interaction with Leshy followed by a new multi wave battle that wasn't required before, 5 seconds added by being forced to talk to the fisherman before continuing, Leshy sacrifice and transformation scene that adds 23 seconds before the fight begins.

First 3 Runs: Addition of having to choose a prize, wait for the chest to spawn and open, move to another room and use the teleport portal every round as opposed as killing boss, redeeming person, and going straight to the result screen adding roughly 13 to 15 seconds per round.

Edited for Clarification: I am currently working on Leshy% and these are the delays I have noticed JUST in that first Darkenwood segment.