Skip Helicopter Stun: This probably doesn't save any time but hey, it's there if you want to use it

Safe Library Ladder [Leon/Claire A]: Before setting foot on the ground, climb back up and it will trigger the zombies to grab you but they can't and they'll pass right through.

Faster Item Extraction from Examine [Controller only]: Use the right analog stick to zoom the item out. You're getting more coverage of the rotation even though it looks like the speed hasn't changed.

Picking up keys mid quick turn [Leon A/Claire A & B]: This method should work on PC. As you approach the keys, turn your character sideways, hit Quick Turn as you approach the door (on PC this would be letting go of W then A or D), then press the Confirm button to pick up the keys DURING the turn animation. Here, you can see me doing it so smooth that you don't even see the animation.

Lab Lickers [Leon/Claire A]: After dodging the 1st and 2nd licker, approach the table and throw the Flash Grenade so that it bounces off of the bottom portion. If done correctly, the flash will trigger sooner, knocking the lickers out without having to turn around.