[Mod note]:

Split very early (19.667) and early at the end (2.166). Final time should be 39:53.8 - 19.6 = 39.34.2. Congrats on the run.

Split when you gain total of the turtle and can move (when the screen transitions from being black and you can move after the "Fire!" scene) and end the splits when the screen starts shaking once Shredder dies. :)