Friend of mine found a way to hit the button for the Bayou/Caviar gate from the wrong side.

I think that's a great idea. I'd certainly take a stab at it considering the number of times I've forgotten to equip or level up an evolution. lol

Honestly I've been pondering over a DLC run myself but I don't have the time currently to sort out efficiency. Could probably have a couple of categories; speedrun game & DLC both any% & 100%, and just the DLC any% & 100%.