RedDragon, I ran it through shazam and got "The Barber of Seville: Overture"

Yoooo it's an actual category now pog

Update to this category. Turns out on Medium difficulty, you can save time by picking up the rotten Larvae blade and using that instead of crafting/analyzing anything, but this doesn't work on Woah! Difficulty because the weapon you use in this pathing just doesn't have enough durability. Unfortunately, this run is actually harder than Woah! difficulty, mainly because there's a Larvae in the area and it's easier to break the weapon. That being said, I shaved off almost a full minute and wound up with this run here.

Hey, I was wondering if this could be a new speedrun category: Defeating a Wolf Spider (on Woah! difficulty) as fast as possible after starting the game.

I decided to do some runs of this already and here's my current best When Starting the timer after hitting "Start game" with a character, I managed to get 3m52s. This still has plenty of room for improvement so I think it could be a fun category