You don't have to do a certain route for a category but there are certain checkpoints/guidelines you have to do in categories, like you can't SBLJ in 16 star you have to use Mips.
If you are new you should try those beginner and/or advanced to have a good basis to go off of, and start watching other speedrunners, near/above the skill level you might be at, and figure out what tricks you can do at that time and optimize your route to your liking.
you can do any route as long as it follows the rules of the category
If you wish to replace your twitch highlights with reuploads, please edit your already verified run rather than deleting the verified run and making a new submission. This makes it a lot easier for us to track which submissions are reuploads and should be less work on your part as well.
If you need