Trying to figure out what routes to take for the run. So far I've been starting with Pyro and working through based on which stages have advice for them. I'm considering starting a run on Bat and seeing if his weapon can speed up my run, especially since I don't use Pyro's or Cryo's weapons for most of the run. Here's my current level order:

City No. 1 No. 2 No. 5 No. 4 No. 7 No. 3 No. 8 No. 6 Prison Factory Coliseum

Haven't timed any runs, especially since I don't know what would start or end the timer, but I'll start getting vague numbers soon. I've been able to cut my run down to under 3 hours, but I know it can be run faster. Looking forward to seeing if a speedrunning community develops for this game.

I've had luck with 5-4-7-3-8-6-1-2-Prison-Factory-Coliseum but I think it varies pretty wildly.

in reality going with 5 first will speed your game up because his weapon is the strongest weapon and will allow the I-frame glitch to work early game