Seed: 1.4.1.836959164
I FINALLY DID IT LET'S GO!!!ヾ(^▽^*))) This run has a lot of weird and dumb time losses, and I did the bosses in an odd order, but I got a truffle worm, and that's all that matters in this category. Also, the jungle was insanely good. Sub-40 is absolutely possible; maybe even sub-35.
Timestamps
0:00 - World and Character Creation 0:59 - Wall Of Flesh 8:56 - Spazmatism 11:09 - The Destroyer 11:49 - Skeletron Prime 13:13 - Eye of Cthulhu 14:25 - Plantera 16:34 - Golem 19:23 - Skeletron 21:04 - Lunatic Cultist 21:54 - Moon Lord 33:00 - Queen Slime 34:07 - Truffle Worm (Hardest Boss) 37:26 - Empress of Light 39:34 - Queen Bee 40:38 - Eater of Worlds 42:24 - King Slime 42:41 - Duke Fishron 43:36 - End of Run
note: retimed to 42:37