i use mGBA 0.10.3 Gameboy Advance emulator. Timer started at Scrat 1 entry and stop at the end of ellie's final box. Bosses RNG are random can't tell when the fish gonna spawn and can't tell when the World 3 and 5 boss throws stuff. The Game music was muted to reduce the slowdown at some spots in any stage. tested that on my GBA SP. it's why i'm playing music from SNES SPC700 Player Playlist outside the game.

Mod-Note (NinjaCocktail): Retimed 2:24:51