I want to ask a question about dlc，the rules write“DLCs that give 1 copy of cards are banned” that means can't use the DLC？when I start the story，I must be TD02 only to start?

Yes, I meant these DLCs where you have cards for clans.

Sadly I don't have any of these DLCs myself and I'm not sure if it's possible to disable them if you have it. If you can't disable them I'll need to look for some solution but allowing it is out of the question since that would make the speedrun Pay to win :(