My first big run that I've recorded. There are several minor time losses and I know it can definitely be beaten.
In one of my previous runs, I got a time faster than this by about 10 seconds. The most major areas of time loss are the bosses and their strange AI, especially the guy with the energy shield.
The spider queen can be sniped and doesn't require you to kill her goons, they'll still come after you though. Her shotgun is very dangerous so be careful about getting close.
Ryder can be baited into a shotgun blast like you see in this video for a fast kill, or if that doesn't work you can drive in a circle to avoid shots from enemies while you deal damage on him.
The energy shield guy is the most frustrating, and I haven't figured out a consistent strategy to quickly beat him yet.
All enemies will spawn in the exact same places every time - the only RNG is in the ways they act. For example it's good to kill the spider pack on the way to the first town to get enough to buy a speeder - you'll see in this video that I miss them and have to go back to find them.
Link to my recording: