I was doing a 20 achievements, no buildings run and accidentally exited off of my game, I immediately reopened it afterwards and continued where I left off just in case the run was still valid. I am assuming the run is invalid because of this but just wanted to confirm so I don't delete the footage of my run or anything.

I'd say just submit it anyways and see what they say. Maybe also ask on the discord too for a faster response?

No it does not

i did it a couple of times and it's still valid

It's fine.

it is cool if you dont abuse changing account or nothing, as long as it kept recording indeed, i have reseted the page with f5 before for alterating golden cookies chance for neverclick

It's fine