Since I ain't playing anything else really right now, I've been trying to find what helps make the run faster. Here's what we have:

1. Turning off Blinking lights / Fast animation option setting skips the weird flashing scene when you start a new file. Saves about 8 seconds.

2. The more recent discovery is pressing B during screen transitions. Apparently this skips or speeds up the transitions from doors. Unknown from as a whole, but each transition can save roughly a second for each transition.

Another find that I discovered just randomly after doing a reset is that during the third room during the tutorial where the game forces you to reset the room for the platforms to appear, you can reset that room, exit back to main menu, and start the game again from the beginning. By setting this up prior to a run, you can have it set specifically to have those platforms spawn in the room without having to reset the room.