Here is the list of things I found while trying this game:

1. Pressing the Escape key in the first cutscene will end it quicker. It will also skip any dialog boxes.

2. Pressing space/enter is equivalent to selecting ok.

3. When you get to bay 6, bump an island after using the afterburner to get out of the tutorial faster.

4. As for my speedrun (Any% and 100%), I will upload it later.

5. How do you have Livesplit beside Turtle Bay? Did you use a second screen, because I can't make the game windowed. Also, there is no option to use VSync, so it runs at like 140+ fps.

6. In the options, there is a difficulty selection: (Easy, Medium, Hard). Does each of these options affect the gameplay?

Additionally, in 100%, in bonus bay 10, bomb at least 1 octopus, otherwise, the game will be softlocked.

Other notes (not from me) can be found here: https://www.speedrun.com/turtle_bay/runs/zp3vwqgm

From note 7 (Reply): Actually, you can. However, you need at least 6 hearts for each bomb since it deals 5 hearts of damage i think.