Since Duckstation runs are timed in real time, we want to be more precise about the timing so that we can remove any human error from starting/stopping splits. Timing will start and stop on specific frames:

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Start
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New Game - Time starts on the first frame of the screen turning white after selecting difficulty.

New Game + - Time starts on the first frame of the textbox after selecting Yes/はい to load your save. (https://imgur.com/a/SJaoGz4 is the Japanese text).

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End
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Time ends on the first frame after the Weapon/Bullet/Condition HUD disappears upon defeating Hypnos 3.

We will be retiming existing runs over the next few days, which will take some time, so please be patient!

It is strongly suggested and requested that you upload Duckstation runs to YouTube to aid in verification. You can easily get precise timings of YouTube videos using the tool below:
https://slashinfty.github.io/yt-frame-timer/