Like if I got a 5:03 but I didn't use any glitches, could I upload it to both Any% and Glitchless?

Yes.

Thanks!

just to add on would fast accel be considered a glitch?

fast accel isn't currently considered a glitch (since the devs most likely intended for mario to accelerate faster when facing the opposite direction)

Fast acceleration is not a glitch, it's a game mechanic.

But why the world record(for glitchless) didn't do the fast acceleration???

Probably because it's just hard to do and doesn't save many framerules, I'm pretty sure it's only been done in one WR ever (at least in any%).

Any framerule improvement would be major, but no WR runs have used it effectively in 8-1 or 4-2, as it is (currently) too difficult for RTA.

Oh ok. And I know it's hard. The only WR I know that uses the fast acceleration (in 8-4)is by kosmicd12's 4:56.462.

@TheSpenzers I think that somewes also did fast acceleration in his 4:56.245.