Hello guys, today I tested the route and feasibility of a possible Knife Only category. I was able to finish a segmented run, the hardest part is not what you may think, that is the 2nd Boss the Witch and/or the 3rd Boss the Demon, but rather those big fast animal-like enemies you find in the game after killing the Witch, lots of First Aid Boxes get wasted becasue of them. You need to spare at least 1 for the first boss, 2 for the Witch and 2 for the Demon. it might be frustrating, but I think it's surely possible to end a run in a Single Segment. I have recorder my boss fights:

The Witch:

The Demon:

I would not open the category until someone proves this possible. I may try in the future... maybe. I hope someone will eventually.