Few things changed.

There is some "hardcore" mode that you can select before going into the game where if your characters dies, they can't re-spawn and you lose them. If the one of characters from the main story die, you cannot continue the game. I did not select this.

There is also an option to skip the introductory part of intro%, getting Horatio, Pip, Youseff, Sofia and the cupcake. I did not skip this.

I am not sure if I have been getting unlucky or does it seem that Horatio takes 2 hits to hit the last enemy now... I remember last time there was some rng basis, this may have changed or I am just getting really bad RNG.

Sofia map layout has changed where 1 tile has been changed so that neither you or the enemy can go on it.

Sofia map enemies seem to move in a different manner(???) maybe just rng.

However, the cost of buying the 2 extra character slots is much cheaper now. There is no need to sell items, you should already have enough money already. (This means you could have enough money to buy nitro to speed up your vehicle. I still can't really control my vehicle using nitro though.

Extra 2 story missions after the helmet boss.

And I think that is it....