Submitting this to force me to do a better one! Big thank you to Hi5 for introducing me to these runs :)

VERIFIER NOTE:

The following are frame timed using somewes.com at 29.96299934387207 fps (suggested for Twitch VOD).

KELP FOREST

Start Time (New Game), End Time (Loading Triangle Appears)

Start Time 35.844, End Time: 3:53.989, Time: 3:18.144

+1:15 CHAPTER SELECT = 4:33.144

CORAL REEF

Start Time (Door Closed), End Time (Loading Triangle Appears)

Start Time 4:40.279, End Time: 10:56.343, Time: 6:16.064

OLD TEMPLE

Start Time (Door Closed), End Time (Loading Triangle Appears)

Start Time 11:41.966, End Time: 16:26.951, Time: 4:44.985

ABYSS RIFT

Start Time (Door Closed), End Time (Loading Triangle Appears)

Start Time 17:01.26, End Time: 23:06.877, Time: 6:05.618

FACTORY SHIP

Start Time (Door Closed), End Time (Loading Triangle Appears)

Start Time 23:21.762, End Time: 25:36.996, Time: 2:15.233

ATLANTIS CITY

Start Time (Diver Leaves Ground and Flipper Appears), End Time (Loading Triangle Appears)

Start Time 26:35.301, End Time: 33:31.314, Time: 6:56.013

PARADISE WATERS

Start Time (Diver Starts Swimming), End Time (End White Light)

Start Time 34:05.489, End Time: 38:46.536, Time: 4:41.047

LRT: 35 minutes, 32.104 seconds